Mitochondrial Energy Metabolism in Baby Hamster Kidney (BHK-21/C13) Cells Treated with Karnozin EXTRA®

Carnosine is known as a natural dipeptide, which inhibits the proliferation of tumor cells throughout its action on mitochondrial respiration and cell glycolysis. However, not much about effects metabolism healthy cells. We explored Karnozin EXTRA® capsule with different concentrations L-carnosine, viability expressions intermediate filament vimentin (VIM) superoxide dismutase (SOD2) in normal fibroblasts BHK-21/C13. Furthermore, we investigated energy production these Cell was quantified by MTT assay. The Clark oxygen electrode (Oxygraph, Hansatech Instruments, England) used to measure “intact rate”, state 3 ADP-stimulated oxidation, maximum oxidation capacity activities complexes I, II IV. Results showed that 2 5 mM L-carnosine did induce toxic morphological changes treated Our data revealed dose-dependent immunofluorescent signal amplification VIM SOD2 BHK-21/C13 line. This supplement substantially increased recorded rates examined Due stimulation fibroblasts, our results suggested potentially protective dietary prevention diseases altered function.